鐢�(ch菐n)鍝佸悕绋�(ch膿ng) | SHG44-GFP鐔掑厜妯�(bi膩o)瑷樼窗(x矛)鑳炴牚 |
鑻辨枃绨�(ji菐n)绋�(ch膿ng) | SHG44-GFP |
鍍�(ji脿)鏍� | 渚�(l谩i)闆诲彲浜彈鍎�(y艒u)鎯� |
鐢�(ch菐n)鍝佸悕绋�(ch膿ng)锛歋HG44-GFP鐔掑厜妯�(bi膩o)瑷樼窗(x矛)鑳炴牚
瑕�(gu墨)鏍硷細70%瀵嗗害鐨�25cm2鍩归(y菐ng)鐡剁殑绱�(x矛)鑳炰竴鐡�
鍩归(y菐ng)鍩猴細绱�(x矛)鑳炰唬鏁�(sh霉)鐐�4浠d互?x煤n)?n猫i)
鍖呰锛氬収(n猫i)灞ょ劇(w煤)鑿岃嚜灏佽锛涘皥(zhu膩n)鐢ㄩ槻澹撴场娌洅锛涘灞ら槻澹撲繚婧埃娉¤锛涜(shu艒)鏄庢浉(sh奴)
绱�(x矛)鑳炰締(l谩i)婧愶細ATCC銆乻ciencell銆両CLC
璨ㄦ湡锛�1-2鍛�
閬�(y霉n)杓告柟寮忥細蹇仦閬�(y霉n)杓�
涓€銆佽€愯棩鏍湇鍕�(w霉)浠嬬垂
鑰愯棩绱�(x矛)鑳炴牚涓€鑸噰鐢ㄩ珨澶栦綆婵冨害姊害閬炲鑱�(li谩n)鍚堝ぇ鍔戦噺闁撴柗娌栨搳鏂规硶瑾樺皫(d菐o)鑵槫绱�(x矛)鑳炲皪(du矛)鐗瑰畾钘ョ墿鐢�(ch菐n)鐢熻€愯棩鎬�锛屽緸鑰屽缓绔嬬浉鎳�(y墨ng)鐨勮棩鐗╄€愯棩绱�(x矛)鑳炴牚銆�
浜�銆佽€愯棩鏍湇鍕�(w霉)鍏�(n猫i)瀹�
1. 楂斿浣庢績搴︽搴﹂仦澧炶伅(li谩n)鍚堝ぇ鍔戦噺闁撴柗娌栨搳鏂规硶瑾樺皫(d菐o)绱�(x矛)鑳炵殑鑰愯棩
2. MTT娉曟脯(c猫)瀹氳棩鐗╁皪(du矛)瑕湰绱�(x矛)鑳炲拰鑰愯棩绱�(x矛)鑳炵殑IC50锛岃▓(j矛)绠楄€愯棩鎸囨暩(sh霉)
涓�銆佽€愯棩鏍湇鍕�(w霉)瑾�(shu艒)鏄�
1. 瀹㈡埗(h霉)鎻愪緵锛氱敓闀�(zh菐ng)鐙€娉佽壇濂界殑瀹夸富绱�(x矛)鑳炴牚锛屽苟鎻愪緵绱�(x矛)鑳炵壒鎬�锛屽煿椁�(y菐ng)姊濅欢鍙婄浉闂�(gu膩n)娉ㄦ剰浜嬮爡(xi脿ng)銆�
2. 瀹㈡埗(h霉)鎻愪緵鐩搁棞(gu膩n)瑾樺皫(d菐o)鍔�
鍥�銆佺祼(ji茅)鏋滀氦浠�
1. 鎻愪氦瀵�(sh铆)椹�(y脿n)鍫�(b脿o)鍛婃浉(sh奴)锛堣€愯棩鎸囨暩(sh霉)銆佸(sh铆)椹�(y脿n)瑭﹀姂鑸囪ō(sh猫)鍌�銆佸(sh铆)椹�(y脿n)閬�(gu貌)绋�銆佺祼(ji茅)鏋滃垎鏋�銆佸師濮嬫暩(sh霉)鎿�(j霉)銆佺窗(x矛)鑳炲湒鐗囩瓑锛�
2. 鑰愯棩绱�(x矛)鑳炴牚銆佽Κ鏈窗(x矛)鑳炴牚
鑰愯棩绱�(x矛)鑳炴牚鐨勫缓绔嬶細
閲囩敤楂斿浣庢績搴﹀煿椁�(y菐ng)绱�(x矛)鑳�锛岄€愭鍔犻噺瑾樺皫(d菐o)鑵槫绱�(x矛)鑳炲皪(du矛)鐗瑰畾钘ョ墿鐢�(ch菐n)鐢熻€愯棩鎬э紝寰炶€屽缓绔嬬浉鎳�(y墨ng)鐨勮棩鐗╄€愯棩绱�(x矛)鑳炴牚锛堟績搴﹂仦澧炴硶锛�銆�
绱�(x矛)鑳炶€愯棩鎬э細
CellTiter-Glo 鐧�(f膩)鍏夋硶锛圕TG锛夋脯(c猫)瀹氳棩鐗╁皪(du矛)瑕湰绱�(x矛)鑳炲拰鑰愯棩绱�(x矛)鑳炵殑IC50锛岃▓(j矛)绠楄€愯棩鎸囨暩(sh霉)(RI)銆�
RI=鑰愯棩绱�(x矛)鑳濱C50锛忚Κ浠g窗(x矛)鑳濱C50銆�
鍊掔疆椤井閺¤瀵熺窗(x矛)鑳炲舰鎱�(t脿i)瀛�(xu茅)璁婂寲
娓�(c猫)瀹氬€嶅鏅�(sh铆)闁撱€佺躬鍒剁敓闀�(zh菐ng)鏇茬窔
WB銆丳CR銆佺窗(x矛)鑳炪€佸厤鐤瓑澶氶爡(xi脿ng)妾㈡脯(c猫)
瀹㈡埗(h霉)鎻愪緵锛�
1銆佽Κ鏈牚绱�(x矛)鑳炲悕绋�(ch膿ng)銆佸煿椁�(y菐ng)姊濅欢锛�
2銆佽│鍔戠殑**璨ㄨ櫉(h脿o)鑸囦締(l谩i)婧愶紱